cd47 monoclonal antibody  (MedChemExpress)

Journal: Theranostics

Article Title: Macrophage-biomimetic nanomedicine for targeted therapy of abdominal aortic aneurysm via Nrf2/NF-κB pathway

doi: 10.7150/thno.123428

Figure Lengend Snippet: Characterization of MPB-RLZ@MM NPs. ( A ) Schematic diagram depicting the synthesis of MPB-RLZ@MM NPs. ( B-C ) TEM images of MPB and MPB-RLZ NPs. Scale bar = 100 nm. ( D ) XRD pattern of MPB and MPB-RLZ NPs. ( E ) Raman spectra of MPB and MPB-RLZ NPs. ( F ) UV-vis spectra of MPB NPs, RLZ, and MPB-RLZ NPs. ( G ) FTIR spectra of MPB NPs, RLZ, and MPB-RLZ NPs. ( H ) XPS spectra of MPB and MPB-RLZ NPs. ( I ) N 2 adsorption-desorption isotherms of MPB and MPB-RLZ NPs. ( J ) TEM image of MPB-RLZ@MM NPs. Scale bar = 100 nm. ( K ) SEM image of MPB-RLZ@MM NPs. Scale bar = 200 nm. ( L ) Average geometric particle size of the nanoparticles. MPB NPs (n = 44) vs MPB-RLZ NPs (n = 55) vs MPB-RLZ@MM (MRM) NPs (n = 41). Data were presented as mean ± SD. ( M-N ) Zeta potential and PDI of the nanoparticles (n = 3, mean ± SD). ( O ) Elemental mapping of MPB-RLZ@MM NPs. Scale bar = 100 nm. ( P ) Western blotting analysis of CD47 and integrin α4β1 protein expression. I: macrophages; II: macrophage membrane; III: MPB-RLZ@MM NPs; IV: MPB-RLZ NPs. ( Q ) Cumulative release of free RLZ from MPB-RLZ@MM NPs in PBS or H 2 O 2 buffer (n = 3, mean ± SD).

Article Snippet: CD47 monoclonal antibody was supplied by Proteintech Group, Inc. (Wuhan).

Techniques: Adsorption, Zeta Potential Analyzer, Western Blot, Expressing, Membrane

Journal: European Journal of Microbiology & Immunology

Article Title: CD47 monoclonal antibodies differ in their capacity to induce immune response

doi: 10.1556/1886.2025.00065

Figure Lengend Snippet: Anti-CD47 clone MIAP301 treatment induces stronger myeloid immune activation. C57BL/6 mice were treated with anti-CD47 clone MIAP410, anti-CD47 clone MIAP301, or isotype control i.p. (100 µg/mouse, daily) for two days. Mice were infected with 2 × 10 6 PFU/200 µL LCMV intravenously. (A) RT-PCR analysis for IFNα4, IFNβ1, and Mx1 was performed on RNA extracted from the spleen. (B) Total circulating CD11b + , CD11b + Ly6C, and CD11 + circulating cells in blood. (C) Percentage of CD11b + Cy3 + cells in mice treated with anti-CD47 MIAP410 or MIAP301 or isotype infected with 2 × 10 6 LCMV-Cy3 i.v. The data shown was confirmed in two independent experiments ( n = 6) and is shown as mean ± SEM. The statistical comparison of infected vs. control mice was performed using Student's t -test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)

Article Snippet: Anti-CD47 monoclonal antibodies clone MIAP410 (Cat no: BE0283) and MIAP301 (Cat no: BE0270) used in this study were purchased from Bio X Cell.

Techniques: Activation Assay, Control, Infection, Reverse Transcription Polymerase Chain Reaction, Comparison

Journal: European Journal of Microbiology & Immunology

Article Title: CD47 monoclonal antibodies differ in their capacity to induce immune response

doi: 10.1556/1886.2025.00065

Figure Lengend Snippet: Treatment of MIAP301 and MIAP410 induces an inconsequential difference in NK cell response. C57BL/6 mice were treated with anti-CD47 MIAP410 or MIAP301, or isotype control i.p. (100 µg/mouse, daily) for 2 days and were infected with LCMV for 24 h. (A) Gating strategies of splenic NK cells and (B) percentage of NK1.1 + cells. Intracellular cytokine staining of IFN-γ, perforin, and granzyme B on splenocytes. Percentage of (C) perforin + and granzyme B + NK cells, (D) IFN-γ + NK cells, and (E) CD107a + NK cells. The data shown was confirmed in two independent experiments ( n = 6) and is shown as mean ± SEM. The statistical comparison of infected vs. control mice was performed using Student's t -test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)

Article Snippet: Anti-CD47 monoclonal antibodies clone MIAP410 (Cat no: BE0283) and MIAP301 (Cat no: BE0270) used in this study were purchased from Bio X Cell.

Techniques: Control, Infection, Staining, Comparison

Journal: European Journal of Microbiology & Immunology

Article Title: CD47 monoclonal antibodies differ in their capacity to induce immune response

doi: 10.1556/1886.2025.00065

Figure Lengend Snippet: MIAP301 and MIAP410 treatment led to a subtle difference in T cell activation and function. C57BL/6 mice were treated with anti-CD47 MIAP410 or MIAP301 or isotype control i.p. (100 µg/mouse, daily) for −2, 0, 1, and 4 days and infected with LCMV for 8 days. (A) Gating strategies of CD8 + T cells from blood. (B) Total circulating CD8 + T cells and LCMV-gp33-specific CD8 + T cells in the blood. (C) Total CD4 + T cells in the blood. Percentage of (D) IFN-γ + CD8 + T cells and (E) IFN-γ + CD4 + T cells from splenocytes. The data shown was confirmed in two independent experiments ( n = 6) and is shown as mean ± SEM. The statistical comparison of infected vs. control mice was performed using Student's t -test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)

Article Snippet: Anti-CD47 monoclonal antibodies clone MIAP410 (Cat no: BE0283) and MIAP301 (Cat no: BE0270) used in this study were purchased from Bio X Cell.

Techniques: Activation Assay, Control, Infection, Comparison